Coxsackie B virus

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Coxsackie B (also written coxsackievirus B) is a group of six types of enterovirus belonging to the Picornaviridae family. They cause symptoms ranging from gastrointestinal distress to aseptic meningitis, pericarditis and myocarditis. Like other enteroviruses, Coxsackie B viruses have a tropism for muscle cells and have been linked to myalgic encephalomyelitis and chronic fatigue syndrome, fibromyalgia,[1] as well as Type 1 Diabetes.

Symptoms[edit | edit source]

Symptoms of infection with viruses in the Coxsackie B grouping include fever, headache, sore throat, gastrointestinal distress, extreme fatigue as well as chest and muscle pain. It can also lead to spasms in arms and legs.

Types[edit | edit source]

Coxsackie B1[edit | edit source]

Coxsackie B2[edit | edit source]

Coxsackie B3[edit | edit source]

Coxsackie B3 is found in 20-25% of patients with cardiomyopathy and myocarditis.[2][3][4][5]

Coxsackie B4[edit | edit source]

Coxsackievirus B4 has a cell tropism for natural killer cells and pancreatic islet cells.[citation needed]

Coxsackie B5[edit | edit source]

Coxcackie B6[edit | edit source]

Immune system[edit | edit source]

In a mouse model of myocarditis, Coxsackievirus infection was found to upregulate Toll-like receptor 4 on mast cells and macrophages immediately following infection. It also increased numbers of mast cells.[6]

The induction of interferon signaling and the induction of apoptosis are required for normal control of a Coxsackie B3 infection. Coxsackievirus B3 cleaves Mitochondrial Antiviral Signaling (MAVS) protein and Toll/IL-1 receptor domain-containing adaptor inducing interferon-beta TRIF to inhibit type I interferon induction and evade host immunity.[7] Conversely, upregulation of MAVS inhibits Coxsackie B3 by increasing type-1 interferon production.[8]

Mitochondria[edit | edit source]

Coxsackievirus B3 cleaves Mitochondrial Antiviral Signaling (MAVS) protein to inhibit type I interferon induction.[9] Conversely, upregulation of MAVS inhibits coxsackie B3 by increasing type-1 interferon production.[10]

Exercise[edit | edit source]

Several studies of a mouse model of Coxsackie B3 myocarditis have found that exercise increases the virulence of the infection and results in poorer outcomes.[11][12][13][14][15] These studies compare two groups of mice, both infected with CVB3, one that is exercised and the other, sedentary. They found:

  • Exercised mice died of congestive heart failure (the majority while swimming) and had 530X the amount of virus.[14]
  • Exercised mice had increased viral titers, mortality and fiber necrosis.[12]
  • Exercised mice had higher viremia and virus in the hearts and no circulating interferon; non-exercised mice had detectable interferon activity, higher levels of neutralizing antibodies[15]
  • Exercised mice died at much higher rates (52% v. 0 sedentary mice), but not if they were immunosuppressed.[11]
  • Increased T cytotoxic, T suppressor, and T cytotoxic, suppressor/T helper cell ratio, and myocardial inflammatory and necrotic lesions with exercise at 48 hours after infection. "Failure to restrict physical activity in the acute phase of this infection may well contribute to the progression of the disease."[13]

Chronic infection[edit | edit source]

Coxsackievirus B is able to establish a chronic intracellular non-cytolytic infection which can persist for years. Non-cytolytic enterovirus infection does not involve the destruction of infected cells. Non-cytolytic infection is difficult to measure in the serum as viral particles remain in the cell walls of tissues.

The molecular mechanisms of non-cytolytic infection were examined in a small study comparing Coxsackie B2 virus cultured in vitro to RNA extracted via muscle biopsy from eight patients with a chronic fatigue syndrome diagnosis. All patients had symptoms of muscle fatiguability. Four of these samples tested positive for enteroviral RNA. In all four patients with enteroviral-specific RNA, the enteroviral RNA had equal amounts of positive sense and negative sense RNA. By contrast, CVB2 virus in culture produced positive sense RNA at a ratio of 100:1. An equal ratio of positive to negative sense RNA would inhibit the translation of virus-specific gene products, explaining the failure to attract a response from the host immune system, and my account for how CVB2 could establish a persistent infection in these four patients.[16]

Models of persistent infection of the heart[17] and brain[18] have also been studied in mice and in thyroid carcinoma.

In human disease[edit | edit source]

Viruses in the Coxsackie B family progress to myocarditis or pericarditis, which can result in permanent heart damage or death. Coxsackie B virus infection may also induce aseptic meningitis. As a group, they are the most common cause of unexpected sudden death, and may account for up to 50% of such cases.[19]

Myalgic Encephalomyelitis[edit | edit source]

Some researchers and clinicians postulate that ME is caused by an enteroviral infection. Several studies have found patients with ME to have persistently elevated levels of Coxsackie B IgM or IgG antibodies, circulating immune complexes containing viral antigen, or presence of enterovirus by PCR or culture, all indicating the possible presence of a persistent infection.[20][21] Others studies failed to find a difference in rates of positivity between patients and controls. Differences in study outcomes may be due to the criteria used to define study cohorts as well as the techniques used.

Blood testing[edit | edit source]

Elevated Coxsackie B antibodies have been found in patients in at least two ME outbreaks.[22][23] In a retrospective cohort study[24] by Melvin Ramsay and Elizabeth Dowsett, 31% of the patients were found to have elevated enteroviral IgM antibody levels. Sixteen of these patients were retested annually over three years and all showed persistently elevated Coxsackie B neutralizing antibody levels and intermittently positive enteroviral IgM, suggesting a persistent infection was present.

Similarly, a study of of 76 patients with postviral fatigue syndrome (PVFS) found that 76% had detectible IgM responses to enteroviruses. 22% had positive cultures (compared to 7% controls) and VP1 antigen was detected in 51%, all pointing to a chronic infection in many post-viral patients.[25] However, a larger study in Scotland of 243 PVFS patients and matched controls found no difference in IgM and IgG positivity between patients and controls.[26]

PCR[edit | edit source]

In a study of serum samples from 100 CFS patients and 100 healthy controls, 42% of patients were positive for Coxsackie B sequences by PCR, compared to only 9% of the comparison group.[27]

Also using PCR, a study of 236 patients by John Chia found enteroviral RNA in 48% of patients as compared to 8% of controls.To date, Chia reports finding enteroviral RNA in 35% of 518 patients.[21]

Muscle biopsy[edit | edit source]

Several muscle biopsy studies have also found the presence of Coxsackie B RNA sequences in CFS patients as compared to controls. A study of 60 post-viral fatigue syndrome patients found 53% had enteroviral RNA in muscle compared to 15% of controls.[28] However, a follow-up study comparing CFS patients to patients with other neuromuscular disorders failed to find a statistically significant difference.[29]

Fibromyalgia[edit | edit source]

Type 1 diabetes[edit | edit source]

Several studies have suggested a relationship between Coxsackie B4 and the onset of Type 1 diabetes.[30][31][32]

A study of patients with Type 1 Diabetes found that Coxsackie B4 was found to infect the β cells in the pancreatic islets of the pancreas and cause inflammation mediated by natural killer cells.[33]

Testing[edit | edit source]

In the United States, ARUP Laboratories offers a serum microneutralization assay that is designed to measure the concentration of serum antibodies to six serotypes of the virus; B1 through B6. This specific assay has been shown to be sensitive for detection of chronic infections in ME patients. A persistent fourfold or greater rise in antibody titer is often found in these patients, which is not often found in healthy controls.

A complement fixation assay for Coxsackie B serotypes is available in the United States from LabCorp and Quest Diagnostics, however this specific type of assay has not been found to be sensitive for the chronic infections found in ME patients.

Antivirals and immunomodulators for coxsackievirus B [edit | edit source]

Treatment of enterovirus-associated ME/CFS with a course of Interferon (or interferon plus the antiviral Ribavirin) over a few months results in dramatic improvements, allowing many severe bedbound ME/CFS patients being able to return to work. However, these improvements achieved with interferon typically only last for a short period of a few months to a year, after which relapse usually occurs.[34] Repeated treatment with interferon is usually not feasible, as patients typically eventually develop antibodies against recombinant interferon, which disables this drug. Thus interferon clearly demonstrates that inhibiting the enteroviruses associated with ME/CFS results in large improvements in symptoms; but interferon is not effective as a cure or long-term treatment of ME/CFS.

Dr John Chia finds interferon works for ME/CFS associated with coxsackievirus B3 and B5, but is not effective against coxsackievirus B4.[35]

The mainstay of Dr Chia's treatment of enterovirus-associated ME/CFS is the immunomodulator oxymatrine, which Chia found in an informal study leads to 30% of ME/CFS patients making a major improvement, and another 20% will make more minor improvements. Dr Chia often adds the immunomodulator inosine to oxymatrine. He also uses the antiviral Epivir (lamivudine) to treat enterovirus ME/CFS.[36]

The immunomodulatory drug Ampligen (rintatolimod) is a treatment that has proven effective for ME/CFS in clinical trials, and in mouse models, Ampligen was found to be protective of coxsackievirus B3 myocarditis.[37]

One ME/CFS patient with coxsackievirus B4 experienced moderate improvement on the antiviral drug Pleconaril for one month, along with a fourfold decrease of antibody titers for CVB4 and echoviruses 7 and 11. However on discontinuation of the drug the patient relapsed about one month later, and did not respond to a further month of treatment. Pleconaril is no longer available for investigational use.[21]

Two potent new antiviral drugs for coxsackievirus B code named Rega Compound A and Rega Compound 17 are expected to become available in around 2021. These two drugs were originally developed by the Rega Institute in Belgium (info here). Dr Chia believes these drugs may be effective treatments for enterovirus-associated ME/CFS.

Selenium deficiency increases the virulence of coxsackievirus B3 infections in a mouse model.[38] Drugs and supplements which may have some mild in vivo antiviral effects (at least in murine studies) against coxsackievirus B include:

sophoridine (Sophora root extract),[39] Arbidol (umifenovir),[40][41] astragaloside IV (from Astragalus membranous),[42] cinnamic acid (a metabolite of cinnamon spice),[43] emodin (from Rheum palmatum),[44] dihydroquercetin (DHQ),[45] arsenic trioxide,[46] Spatholobus suberectus (Ji Xue Teng)[47] and salidroside (from Rhodiola rosea).[48]

Drugs and supplements which have in vitro antiviral effects against coxsackievirus B are listed here (but typically in vitro antiviral activity does not translate to in vivo efficacy when the compound is taken orally, so these compounds are likely to have no therapeutic effect, but may be of interest as lead compounds in the development of new antiviral drugs).

Factors which worsen coxsackievirus B infection[edit | edit source]

exercise has been shown to be a risk factor that often worsens coxsackievirus B infection.[12][13][14][15]

See also[edit | edit source]

References[edit | edit source]

  1. Nash, P.; Chard, M.; Hazleman, B. (Nov 1989). "Chronic coxsackie B infection mimicking primary fibromyalgia". The Journal of Rheumatology. 16 (11): 1506–1508. ISSN 0315-162X. PMID 2557447. 
  2. Jin O, Sole MJ, Butany JW, Chia WK, McLaughlin PR, et al. (1990) Detection of enterovirus RNA in myocardial biopsies from patients with myocarditis and cardiomyopathy using gene amplification by polymerase chain reaction. Circulation 82: 8–16.
  3. Bowles NE, Richardson PJ, Olsen EG, Archard LC (1986) Detection of Coxsackie-B-virus-specific RNA sequences in myocardial biopsy samples from patients with myocarditis and dilated cardiomyopathy. Lancet 1: 1120–1123.
  4. Martin AB, Webber S, Fricker FJ, Jaffe R, Demmler G, et al. (1994) Acute myocarditis. Rapid diagnosis by PCR in children. Circulation 90: 330–339.
  5. Jin O, Sole MJ, Butany JW, Chia WK, McLaughlin PR, et al. (1990) Detection of enterovirus RNA in myocardial biopsies from patients with myocarditis and cardiomyopathy using gene amplification by polymerase chain reaction. Circulation 82: 8–16.
  6. http://www.ncbi.nlm.nih.gov/pubmed/15386590
  7. Mukherjee, A (March 2011). "The coxsackievirus B 3C protease cleaves MAVS and TRIF to attenuate host type I interferon and apoptotic signaling". PLoS Pathology. 7. 
  8. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546859/
  9. Mukherjee, A (March 2011). "The coxsackievirus B 3C protease cleaves MAVS and TRIF to attenuate host type I interferon and apoptotic signaling". PLoS Pathology. 7. 
  10. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546859/
  11. 11.011.1 Cabinian AE, Kiel RJ, Smith F, Ho KL, Khatib R, Reyes MR. Modification of exercise-aggravated coxsackie virus B3 murine myocarditis by T-lymphocyte suppression in an inbred model. J. Lab. Clin. Med. 1990; 115: 454– 62.
  12. 12.012.112.2 Kiel RJ, Smith FE, Chason J, Khatib R, Reyes MD. Coxsackie B3 myocarditis in C3H/HeJ mice: Description of an inbred model and the effect of exercise on the virulence. Eur. J. Epidemiol. 1989; 5: 248– 67.
  13. 13.013.113.2 Ilbäck, NG (June 1989). "Exercise in coxsackie B3 myocarditis: Effects on heart lymphocyte subpopulations and the inflammatory reaction". American Heart Journal. 117: 1298–302. 
  14. 14.014.114.2 Gatmaitan, Bienvenido (Jun 1, 1970). "Augmentation of the Virulence of Murine Coxsackie Virus B-3 Myocardiopathy by Exercise". Journal of Experimental Medicine. 131: 1121. 
  15. 15.015.115.2 Reyes, MP (February 1976). "Interferon and neutralizing antibody in sera of exercised mice with coxsackievirus B-3 myocarditis". Proceedings of the Society for Experimental Biology and Medicine. 151: 333–8. 
  16. Cunningham, Louise (1990). "Persistence of enteroviral RNA in chronic fatigue syndrome is associated with the abnormal production of equal amounts of positive and negative strands of enteroviral RNA". Journal of General Virology. 71: 1399–1402. 
  17. Chapman N.M., Kim K.S. (2008) Persistent Coxsackievirus Infection: Enterovirus Persistence in Chronic Myocarditis and Dilated Cardiomyopathy. In: Tracy S., Oberste M.S., Drescher K.M. (eds) Group B Coxsackieviruses. Current Topics in Microbiology and Immunology, vol 323. Springer, Berlin, Heidelberg
  18. Feuer, Ralph (September 2009). "Viral Persistence and Chronic Immunopathology in the Adult Central Nervous System following Coxsackievirus Infection during the Neonatal Period". Journal of Virology. 83: 9356–9369. 
  19. "Coxsackie B virus". Wikipedia. 
  20. Landay, AL (September 1991). "Chronic fatigue syndrome: clinical condition associated with immune activation". Lancet. 
  21. 21.021.121.2 Chia, John (November 2005). "The role of enterovirus in chronic fatigue syndrome". Journal of Clinical Pathology. 
  22. Fegan, KG; Behan, PO; Bell, EJ (Jun 1, 1983), "Myalgic encephalomyelitis — report of an epidemic", J R Coll Gen Pract, 33 (251): 335–337, PMID 6310104 
  23. Calder, BD; Warnock, PJ (Jan 1984), "Coxsackie B infection in a Scottish general practice", Jrnl Royal Coll Gen Pract, 34 (258): 15–19, PMID 6319691 
  24. Dowsett, EG; Ramsay, AM; McCartney, RA; Bell, EJ (Jul 1, 1990), "Myalgic encephalomyelitis--a persistent enteroviral infection?", Postgraduate Medical Journal, 66 (777): 526–530, doi:10.1136/pgmj.66.777.526, PMID 2170962 
  25. Yousef, G.E. (January 1988). "CHRONIC ENTEROVIRUS INFECTION IN PATIENTS WITH POSTVIRAL FATIGUE SYNDROME". The Lancet. 
  26. Miller, N A (1991). "Antibody to Coxsackie B virus in diagnosing postviral fatigue syndrome". The British Medical Journal. 
  27. Nairn, C (August 1995). "Comparison of coxsackie B neutralisation and enteroviral PCR in chronic fatigue patients". Journal of Medical Virology. 
  28. Gow, JW. "Enteroviral RNA sequences detected by polymerase chain reaction in muscle of patients with postviral fatigue syndrome". British Medical Journal. 
  29. Gow, JW (1994). "Studies on enterovirus in patients with chronic fatigue syndrome". Clin Infect Dis. 18 (Supp 1). 
  30. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1587352/
  31. http://link.springer.com/article/10.1007%2Fs00125-003-1297-z
  32. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057729
  33. http://www.pnas.org/content/104/12/5115.full
  34. Chia, J. K. S. (Nov 1, 2005). "The role of enterovirus in chronic fatigue syndrome". Journal of Clinical Pathology. 58 (11): 1126–1132. doi:10.1136/jcp.2004.020255. ISSN 0021-9746. PMID 16254097. The results of antiviral treatment provided supportive evidence for the pathogenic role of RNA in patients with CFS. 
  35. Chia, John (2009). "Diagnosis and Treatment of Myalgic Encephalomyelitis / Chronic Fatigue Syndrome Associated with Chronic Enterovirus Infection. Presentation at the Invest in ME International ME Conference, London 2009 (available on DVD). Timecode: 42:31". Interestingly enough, my son also has coxsackie B4, and for coxsackie B4, the antibody titer did not change at all, which is what I usually see using ribavirin and interferon; it is ineffective against coxsackie B4. 
  36. McLaughlin, Christine (Feb 18, 2014). ""Could This Really Be Happening"? One Year With Dr. Chia – A Chronic Fatigue Syndrome Patient Reports". 
  37. Padalko, E (January 2004). "The interferon inducer ampligen [poly(I)-poly(C12U)] markedly protects mice against coxsackie B3 virus-induced myocarditis". Antimicrobial Agents and Chemotherapy. 48: 267–74. 
  38. http://jn.nutrition.org/content/130/2/485S.full
  39. Zhang, Yuanyuan; Zhu, Haiyan; Ye, Guan; Huang, Chenggang; Yang, Yingzhen; Chen, Ruizhen; Yu, Yong; Cui, Xiaolan (Mar 20, 2006). "Antiviral effects of sophoridine against coxsackievirus B3 and its pharmacokinetics in rats". Life Sciences. 78 (17): 1998–2005. doi:10.1016/j.lfs.2005.09.034. ISSN 0024-3205. PMID 16309710. 
  40. Shi, L.; Xiong, H.; He, J.; Deng, H.; Li, Q.; Zhong, Q.; Hou, W.; Cheng, L.; Xiao, H. (2007). "Antiviral activity of arbidol against influenza A virus, respiratory syncytial virus, rhinovirus, coxsackie virus and adenovirus in vitro and in vivo". Archives of Virology. 152 (8): 1447–1455. doi:10.1007/s00705-007-0974-5. ISSN 0304-8608. PMID 17497238. 
  41. Zhong, Qiong; Yang, Zhanqiu; Liu, Yuanyuan; Deng, Haiying; Xiao, Hong; Shi, Liqiao; He, Jing (2009). "Antiviral activity of Arbidol against Coxsackie virus B5 in vitro and in vivo". Archives of Virology. 154 (4): 601–607. doi:10.1007/s00705-009-0346-4. ISSN 1432-8798. PMID 19291363. 
  42. Zhang, Yuanyuan; Zhu, Haiyan; Huang, Chenggang; Cui, Xiaolan; Gao, Yingjie; Huang, Yang; Gong, Wenfeng; Zhao, Ye; Guo, Shanshan (Feb 2006). "Astragaloside IV exerts antiviral effects against coxsackievirus B3 by upregulating interferon-gamma". Journal of Cardiovascular Pharmacology. 47 (2): 190–195. doi:10.1097/01.fjc.0000199683.43448.64. ISSN 0160-2446. PMID 16495755. 
  43. Ding, YuanYuan; Qiu, Lin; Zhao, GangTao; Xu, Jingfeng; Wang, Siwang (Aug 2010). "Influence of cinnamaldehyde on viral myocarditis in mice". The American Journal of the Medical Sciences. 340 (2): 114–120. doi:10.1097/MAJ.0b013e3181dd3b43. ISSN 1538-2990. PMID 20460981. 
  44. Liu, Zhao; Wei, Fei; Chen, Liang-Jun; Xiong, Hai-Rong; Liu, Yuan-Yuan; Luo, Fan; Hou, Wei; Xiao, Hong; Yang, Zhan-Qiu (Sep 25, 2013). "In vitro and in vivo studies of the inhibitory effects of emodin isolated from Polygonum cuspidatum on Coxsakievirus B₄". Molecules (Basel, Switzerland). 18 (10): 11842–11858. doi:10.3390/molecules181011842. ISSN 1420-3049. PMID 24071990. 
  45. Galochkina, Anastasia V.; Anikin, Vadim B.; Babkin, Vasily A.; Ostrouhova, Liudmila A.; Zarubaev, Vladimir V. (Apr 2016). "Virus-inhibiting activity of dihydroquercetin, a flavonoid from Larix sibirica, against coxsackievirus B4 in a model of viral pancreatitis". Archives of Virology. 161 (4): 929–938. doi:10.1007/s00705-016-2749-3. ISSN 1432-8798. PMID 26780775. 
  46. Molin, Ylva; Frisk, Peter; Hjelm, Eva; Blomberg, Jonas; Friman, Göran; Ilbäck, Nils-Gunnar (Nov 2010). "Arsenic trioxide influences viral replication in target organs of coxsackievirus B3-infected mice". Microbes and Infection. 12 (12-13): 1027–1034. doi:10.1016/j.micinf.2010.07.003. ISSN 1769-714X. PMID 20638482. 
  47. Pang, Ji; Guo, Jin-peng; Jin, Min; Chen, Zhi-qiang; Wang, Xin-wei; Li, Jun-Wen (Oct 2011). "Antiviral effects of aqueous extract from Spatholobus suberectus Dunn. against coxsackievirus B3 in mice". Chinese Journal of Integrative Medicine. 17 (10): 764–769. doi:10.1007/s11655-011-0642-1. ISSN 1672-0415. PMID 21717161. 
  48. Wang, Haibo; Ding, Yuanyuan; Zhou, Jun; Sun, Xiaoli; Wang, Siwang (Mar 2009). "The in vitro and in vivo antiviral effects of salidroside from Rhodiola rosea L. against coxsackievirus B3". Phytomedicine: International Journal of Phytotherapy and Phytopharmacology. 16 (2-3): 146–155. doi:10.1016/j.phymed.2008.07.013. ISSN 1618-095X. PMID 18818064. 

Antibodies or immunoglobulin refers to any of a large number of specific proteins produced by B cells that act against an antigen in an immune response. [1]

Myalgic encephalomyelitis or M.E. has different diagnostic criteria to chronic fatigue syndrome; neurological symptoms are required but fatigue is an optional symptom.Cite error: Closing </ref> missing for <ref> tag

Myalgic encephalomyelitis or chronic fatigue syndrome, often used when both illnesses are considered the same.

The information provided at this site is not intended to diagnose or treat any illness.
From MEpedia, a crowd-sourced encyclopedia of ME and CFS science and history.