A nanoelectronics-blood-based diagnostic biomarker for myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)

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A biomarker for ME/CFS: using nanoneedles to detect white blood cell changes. Source: Open Medicine Foundation, Apr 29, 2019.

The research aimed to stimulate post-exertional malaise at a cellular level used a high-salt environment to simulate hypersmotic stress white blood cells from patients who met both the Canadian Consensus Criteria for ME/CFS and the more common CDC chronic fatigue syndrome criteria; the test correctly identified all patients with ME/CFS and all healthy controls.

Isolated PBMCs from patient's blood were incubated in their own plasma. Stressing the PBMCs should cause excessive consumption of the high-energy metaboliate ATP. The study investigators stated that this type of salt stress has previously been used in this way in a number of human, animal and other studies.[1]

Funding[edit | edit source]

Part supported by the National Institutes of Health, grant P01 HG000205, and the Open Medicine Foundation.

Abstract[edit | edit source]

There is not currently a well-established, if any, biological test to diagnose myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). The molecular aberrations observed in numerous studies of ME/CFS blood cells offer the opportunity to develop a diagnostic assay from blood samples. Here we developed a nanoelectronics assay designed as an ultrasensitive assay capable of directly measuring biomolecular interactions in real time, at low cost, and in a multiplex format. To pursue the goal of developing a reliable biomarker for ME/CFS and to demonstrate the utility of our platform for point-of-care diagnostics, we validated the array by testing patients with moderate to severe ME/CFS patients and healthy controls. The ME/CFS samples’ response to the hyperosmotic stressor observed as a unique characteristic of the impedance pattern and dramatically different from the response observed among the control samples. We believe the observed robust impedance modulation difference of the samples in response to hyperosmotic stress can potentially provide us with a unique indicator of ME/CFS. Moreover, using supervised machine learning algorithms, we developed a classifier for ME/CFS patients capable of identifying new patients, required for a robust diagnostic tool.[1]

Results[edit | edit source]

Criticism[edit | edit source]

  • Only patients with ME/CFS and healthy controls were compared. The test was not tried for patients with other fatiguing or neurological illness.[citation needed]
  • Small sample size

Investigators[edit | edit source]

Biochemistry research engineer at Stanford,[2] and assistant professor in Electrical Engineering and Computer Science, University of California, Irvine[3]
Stanford Genome Technology Center, Stanford University[2]
Stanford Genome Technology Center and Department of Biochemistry, School of Medicine, Stanford University[4]
Department of Biochemistry, School of Medicine, Stanford University[5]
Stanford Genome Technology Center and Department of Biochemistry, School of Medicine, Stanford University, and Director of the Scientific Advisory Board of the Open Medicine Foundation

Citation[edit | edit source]

Esfandyarpour, R., Kashi, A., Nemat-Gorgani, M., Wilhelmy, J., and Davis, R. W. "A Nanoelectronics-blood-based Diagnostic Biomarker for Myalgic Encephalomyelitis/chronic Fatigue Syndrome (ME/CFS)." Proceedings of the National Academy of Sciences, April 29, 2019, 201901274. doi:10.1073/pnas.1901274116.

Commentary from scientists[edit | edit source]

News articles and blogs[edit | edit source]

Learn more[edit | edit source]

See also[edit | edit source]

References[edit | edit source]

ME/CFS - An acronym that combines myalgic encephalomyelitis with chronic fatigue syndrome. Sometimes they are combined because people have trouble distinguishing one from the other. Sometimes they are combined because people see them as synonyms of each other.

Myalgic encephalomyelitis (ME) - A disease often marked by neurological symptoms, but fatigue is sometimes a symptom as well. Some diagnostic criteria distinguish it from chronic fatigue syndrome, while other diagnostic criteria consider it to be a synonym for chronic fatigue syndrome. A defining characteristic of ME is post-exertional malaise (PEM), or post-exertional neuroimmune exhaustion (PENE), which is a notable exacerbation of symptoms brought on by small exertions. PEM can last for days or weeks. Symptoms can include cognitive impairments, muscle pain (myalgia), trouble remaining upright (orthostatic intolerance), sleep abnormalities, and gastro-intestinal impairments, among others. An estimated 25% of those suffering from ME are housebound or bedbound. The World Health Organization (WHO) classifies ME as a neurological disease.

Myalgic encephalomyelitis (ME) - A disease often marked by neurological symptoms, but fatigue is sometimes a symptom as well. Some diagnostic criteria distinguish it from chronic fatigue syndrome, while other diagnostic criteria consider it to be a synonym for chronic fatigue syndrome. A defining characteristic of ME is post-exertional malaise (PEM), or post-exertional neuroimmune exhaustion (PENE), which is a notable exacerbation of symptoms brought on by small exertions. PEM can last for days or weeks. Symptoms can include cognitive impairments, muscle pain (myalgia), trouble remaining upright (orthostatic intolerance), sleep abnormalities, and gastro-intestinal impairments, among others. An estimated 25% of those suffering from ME are housebound or bedbound. The World Health Organization (WHO) classifies ME as a neurological disease.

The information provided at this site is not intended to diagnose or treat any illness.
From MEpedia, a crowd-sourced encyclopedia of ME and CFS science and history.