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List of enterovirus infection studies
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== Other investigations by John Chia == Dr Chia took more than 2500 blood samples from more than 510 ME/CFS patients, and in their peripheral blood lymphocyte (PBL) cells, 35% of these patients tested positive for enterovirus RNA, using a very sensitive test: the Chemicon RT-PCR EAI 3 primer test, with Qiagen 1-step RT-PCR enzyme. Interestingly, for the more severe bedridden patients, Dr Chia found enterovirus RNA in 70% of the PBL cells. But for the less ill patients, enterovirus RNA was found in only 12%. The sensitivity of the test was high, around 80 to 800 RNA copies per ml of blood, yet typically the same patient would not always test positive using this test (they would be positive on some occasions and negative on others), so Chia says it is clear that the enteroviral RNA present in the blood in ME/CFS is at very low levels.<ref>{{Cite web|url=https://www.youtube.com/watch?v=Xz7CaxWtCUU&t=16m04s | title = Dr John Chia: The Role of Enterovirus in Chronic Fatigue Syndrome / Myalgic Encephalomyelitis. Presentation by Dr John Chia at the International Symposium on Viruses In Chronic Fatigue Syndrome and Post-Viral Fatigue. 2008. Timecode: 16:04. | last = Chia | first = John | date = 2008 | website = |archive-url=|archive-date=|url-status=|access-date=}}</ref> Dr Chia says any PCR tests which have insufficient sensitivity (above 1000 copies of RNA per ml of blood) will almost always give negative results in ME/CFS patients.<ref>{{Cite web|url=http://www.investinme.org/IIMEC10.shtml | title = Dr John Chia: Enteroviruses and Myalgic Encephalomyelitis / Chronic Fatigue Syndrome: An Update on Pathogenesis. Presentation at the Invest in ME International ME Conference, London 2015 (available on DVD). Timecode: 12:00. | last = Chia | first = John | date = |website=|archive-url=|archive-date=|url-status=|access-date=}}</ref>
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