Anonymous
Not logged in
Talk
Contributions
Create account
Log in
Search
Editing
A nanoelectronics-blood-based diagnostic biomarker for myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)
From MEpedia, a crowd-sourced encyclopedia of ME and CFS science and history
Namespaces
Page
Discussion
More
More
Page actions
Read
Edit
Edit source
History
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
{{Video|id=6Qn0fIV8SbE|service=youtube|dimensions=550|description=A biomarker for ME/CFS: using nanoneedles to detect white blood cell changes. Source: [[Open Medicine Foundation]], Apr 29, 2019.|alignment=right|urlargs=start=0&rel=0&autoplay=0}} The research aimed to stimulate [[post-exertional malaise]] at a cellular level used a high-salt environment to simulate hypersmotic stress [[leukocyte|white blood cell]]s from patients who met both the [[Canadian Consensus Criteria]] for [[ME/CFS]] and the more common CDC [[Fukuda criteria|chronic fatigue syndrome criteria]]; the test correctly identified all patients with ME/CFS and all healthy controls.<ref name="Davis2019" /><br> Isolated [[peripheral blood mononuclear cell|PBMC]]s from patient's blood were incubated in their own plasma. Stressing the PBMCs should cause excessive consumption of the high-energy metaboliate [[ATP]]. The study investigators stated that this type of salt stress has previously been used in this way in a number of human, animal and other studies.<ref name="Davis2019" /> ==Funding== Part supported by the [[National Institutes of Health]], grant P01 HG000205, and the [[Open Medicine Foundation]]. ==Abstract == There is not currently a well-established, if any, [[diagnostic biomarker|biological test to diagnose myalgic encephalomyelitis/chronic fatigue syndrome]] (ME/CFS). The molecular aberrations observed in numerous studies of ME/CFS blood cells offer the opportunity to develop a diagnostic assay from blood samples. Here we developed a nanoelectronics assay designed as an ultrasensitive assay capable of directly measuring biomolecular interactions in real time, at low cost, and in a multiplex format. To pursue the goal of developing a reliable biomarker for ME/CFS and to demonstrate the utility of our platform for point-of-care diagnostics, we validated the array by testing patients with moderate to severe ME/CFS patients and healthy controls. The ME/CFS samples’ response to the hyperosmotic stressor observed as a unique characteristic of the impedance pattern and dramatically different from the response observed among the control samples. We believe the observed robust impedance modulation difference of the samples in response to hyperosmotic stress can potentially provide us with a unique indicator of ME/CFS. Moreover, using supervised machine learning algorithms, we developed a classifier for ME/CFS patients capable of identifying new patients, required for a robust diagnostic tool.<ref name="Davis2019">{{Cite journal | last = Davis | first = R.W. | author-link = Ronald Davis | last2 = Wilhelmy | first2 = J. | author-link2 = Julie Wilhelmy | last3 = Nemat-Gorgani | first3 = M. | author-link3 = Mohsen Nemat-Gorgani | last4 = Kashi | first4 = A. | author-link4 = Alex Kashi | last5 = Esfandyarpour | first5 = R. | author-link5 = Rahim Esfandyarpour | date = Apr 25, 2019 | title = A nanoelectronics-blood-based diagnostic biomarker for myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) | url = https://www.pnas.org/content/early/2019/04/24/1901274116 | journal = Proceedings of the National Academy of Sciences|language=en | volume = 116 | issue = 21 | pages = 10250-10257|doi=10.1073/pnas.1901274116|issn=0027-8424|pmid=31036648|quote=|via=}}</ref> ==Results== A nanoneedle was created that was able to accurately test blood samples from a small number of [[ME/CFS]] patients, showing abnormal responses during a biochemical stress test. This formed the first biological model for ME/CFS. ==Criticism== * Only patients with ME/CFS and healthy controls were compared. The test was not tried for patients with other fatiguing or neurological illness. * Small sample size<ref name="Davis2019" /> ==Investigators== *[[Rahim Esfandyarpour]] :Biochemistry research engineer, [[Stanford University]]<ref name="schmed" /> *[[Alex Kashi|Alex Arron Kashi]] :[[Stanford Genome Technology Center]], [[Stanford University]]<ref name="schmed">{{Cite web | url = https://med.stanford.edu/profiles/browse?org=school-of-medicine/biochemistry/stanford-genome-technology-center | title = Browse School of Medicine {{!}} Stanford Medicine Profiles | website = Stanford Medicine | access-date = 2019-05-09}}</ref> *[[Mohsen Nemat-Gorgani|Mohsen Nemat-Gorgani]] :[[Stanford Genome Technology Center]] and Department of Biochemistry, School of Medicine, [[Stanford University]]<ref name="omfng">{{Cite web | url = https://www.omf.ngo/2018/08/15/mohsen-nemat-gorgani/ | title = Meet Mohsen Nemat-Gorgani, PhD, member of the SGTC Team | last = Open Medicine Foundation | first = | author-link = Open Medicine Foundation | date = 2018-08-15 | website = Open Medicine Foundation|language=en-US|archive-url=|archive-date=|url-status= | access-date = 2019-05-09}}</ref> *[[Julie Wilhelmy]] :Department of Biochemistry, School of Medicine, [[Stanford University]]<ref name="omfjw">{{Cite web | url = https://www.omf.ngo/2018/07/25/julie-wilhelmy/ | title = Meet Julie Wilhelmy, ME/CFS Collaborative Research Center at Stanford Team Member | last = Open Medicine Foundation | first = | author-link = Open Medicine Foundation | date = 2018-07-25 | website = Open Medicine Foundation|language=en-US|archive-url=|archive-date=|url-status= | access-date = 2019-05-09}}</ref> *[[Ronald Davis|Ron W. Davis]] :[[Stanford Genome Technology Center]] and Department of Biochemistry, School of Medicine, [[Stanford University]], and Director of the Scientific Advisory Board of the [[Open Medicine Foundation]] ==Citation == Esfandyarpour, R., Kashi, A., Nemat-Gorgani, M., Wilhelmy, J., and Davis, R. W. "A Nanoelectronics-blood-based Diagnostic Biomarker for Myalgic Encephalomyelitis/chronic Fatigue Syndrome (ME/CFS)." ''Proceedings of the National Academy of Sciences'', April 29, 2019, 201901274. doi:10.1073/pnas.1901274116. ==Commentary from scientists== * [https://med.stanford.edu/news/all-news/2019/04/biomarker-for-chronic-fatigue-syndrome-identified.html Press release: Stanford University]<ref name="pressreleaseApr2019">{{Cite web | url = http://med.stanford.edu/news/all-news/2019/04/biomarker-for-chronic-fatigue-syndrome-identified.html | title = Biomarker for chronic fatigue syndrome identified | last = Armitage | first = Hanae | author-link = | date = Apr 2019 | website = Stanford Medical Center|language=en|archive-url=|archive-date=|url-status= | access-date = 2021-11-15}}</ref> * [https://jamanetwork.com/journals/jama/fullarticle/2737657 Biomarker test for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome] - [[American Medical Association]] ==News articles and blogs == * [https://mecfsresearchreview.me/2019/05/08/nanoelectric-device-could-lead-to-a-diagnostic-blood-test-for-me-cfs/ Nanoelectric device could lead to a diagnostic blood test for ME/CFS] - [[Simon McGrath]] * [https://www.healthrising.org/blog/2019/05/07/threading-the-needle-nanoneedle-scores-big-in-first-me-cfs-test/ Threading the needle nanoneedle scores big in first ME/CFS test] - [[Cort Johnson]], Health Rising * [https://www.medscape.com/viewarticle/912336 Medscape: Biomarker May Be a Diagnostic Test for ME/CFS - Medscape] * [https://www.independent.co.uk/news/health/chronic-fatigue-blood-test-me-myalgic-encephalomyelitis-stanford-a8891906.html Blood test for chronic fatigue syndrome could speed diagnosis, study says - The Independent] ==Learn more == * [https://med.stanford.edu/news/all-news/2019/04/biomarker-for-chronic-fatigue-syndrome-identified.html Press release: Stanford University] * [https://www.omf.ngo/2019/04/29/omf-funded-research-nanoelectronics-blood-based-diagnostic-biomarker/ New OMF-funded Research Publication: A Nanoelectronics-blood-based diagnostic biomarker for ME/CFS] * [https://www.youtube.com/watch?v=ZQTURNzSbVY OMF/Ron Davis Research Update - May 2019 (Video)] ==See also == *[[Diagnostic biomarker]] *[[Open Medicine Foundation]] *[[Ron Davis]] *[[Biomedical research]] ==References== {{Reflist}} [[Category:Notable studies]] [[Category:Diagnostic test studies]] [[Category:Seminal works]]
Summary:
Please make sure your edits are consistent with
MEpedia's guidelines
.
By saving changes, you agree to the
Terms of use
, and you irrevocably agree to release your contribution under the
CC BY-SA 3.0 License
and the
GFDL
. You agree that a hyperlink or URL is sufficient attribution under the Creative Commons license.
Cancel
Editing help
(opens in new window)
Templates used on this page:
Template:Cite journal
(
edit
)
Template:Cite web
(
edit
)
Template:Main other
(
edit
)
Template:Reflist
(
edit
)
Template:Video
(
edit
)
Module:Check for unknown parameters
(
edit
)
Module:Citation/CS1
(
edit
)
Module:Citation/CS1/COinS
(
edit
)
Module:Citation/CS1/Configuration
(
edit
)
Module:Citation/CS1/Date validation
(
edit
)
Module:Citation/CS1/Identifiers
(
edit
)
Module:Citation/CS1/Utilities
(
edit
)
Module:Citation/CS1/Whitelist
(
edit
)
Module:No globals
(
edit
)
Navigation
Navigation
Skip to content
Main page
Browse
Become an editor
Random page
Popular pages
Abbreviations
Glossary
About MEpedia
Links for editors
Contents
Guidelines
Recent changes
Pages in need
Search
Help
Wiki tools
Wiki tools
Special pages
Page tools
Page tools
User page tools
More
What links here
Related changes
Page information
Page logs