File:White blood cells in MECFS NK.jpg
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Leucocyte gating strategy for phenotypic characterisation of PBMC. Thawed PBMC from Biobank participants were stained with labelled antibodies (Supplementary Table S2), expression data were collected by Flow cytometry and analysed using FlowJo. (A) Single cells were gated based on their forward scatter height and area, and (B) monocytes within the single cell gate were identified as CD14+. (C) Dendritic cells were identified within the CD3-CD14- cell population as myeloid (CD11c+) or plasmacytoid (CD123+) DCs. (D) Lymphocytes were identified based on their forward and side scatter area profiles, and B cells were identified as CD19+ (E), T cells as CD3+ (F), and NK cells as CD56+ (G). Within the NK cell gate, NK cells were characterised further based on NKG2C (H), NKp46 (I), and CD57 (J) co-expression.
Author: (or citation)[edit | edit source]
Cliff JM, King EC, Lee J-S, Sepúlveda N, Wolf A-S, Kingdon C, Bowman E, Dockrell HM, Nacul L, Lacerda E and Riley EM (2019) Cellular Immune Function in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS). Front. Immunol. 10:796. doi: 10.3389/fimmu.2019.00796
Figure 1
Source: (e.g. internet address)[edit | edit source]
https://www.frontiersin.org/articles/10.3389/fimmu.2019.00796/full
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This file is licensed under the Creative Commons Attribution 4.0 International license. | ||
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current | 13:16, June 27, 2020 | 1,084 × 767 (435 KB) | Notjusttired (talk | contribs) | ==== Title: (or description) ==== Leucocyte gating strategy for phenotypic characterisation of PBMC. Thawed PBMC from Biobank participants were stained with labelled antibodies (Supplementary Table S2), expression data were collected by Flow cytome... |
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